Journal: iScience

Article Title: Targeting B cells in the pre-phase of systemic autoimmunity globally interferes with autoimmune pathology.

doi: 10.1016/j.isci.2021.103076

Figure Lengend Snippet: Figure 4. Effect of pre-phase B cell depletion on humoral immunity (A) Shown is the total amount of serum IgM, IgG1, IgG2b, IgG2c, IgG3 and IgA in 10- and 18-week-old anti-CD20 (aCD20) or isotype control antibody treated female (\) and male (_) BXSB mice as determined by quantitative ELISA. Significant differences between indicated groups were tested with ordinary one-way ANOVA with Tukey’s multiple comparison test (normal distribution) or Kruskal-Wallis plus Dunn’s multiple comparison test (no normal distribution). Two independent experiments with a total of n = 5–12 mice per group. Symbols represent individual mice; error bars represent the mean G SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: For quantification of total serum IgM, IgG and IgA the Mouse IgM, IgG and IgA ELISA Quantification Kits (Bethyl) were used according to the manufacturer’s instructions: ELISA plates were coated with 100 ng/well goat anti-mouse IgM, IgG or IgA in Carbonate/Bicarbonate for 1 h at room temperature.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Nature communications

Article Title: Generation of colonic IgA-secreting cells in the caecal patch.

doi: 10.1038/ncomms4704

Figure Lengend Snippet: Figure 2 | Decreased IgA-secreting cells in large intestinal lamina propria of appendectomized germ-free mice after conventionalization. (a,b) Lamina propria lymphocytes were isolated from appendectomized or sham-operated germ-free(GF) mice at 4 weeks after conventionalization, and stained for surface IgA/B220 or intracellular IL-17/IFN-g/IL-10 in CD4 þ population. (a) Total numbers of IgA þ B220 cells as well as IL-17-, IFN-g- and IL-10-positive CD4 þ cells in the large (upper) and the small intestine (lower) are shown. Each symbol represents an individual mouse, and horizontal bars indicate the mean. (b) Representative dot plots for cells stained with anti-IgA and B220 of appendectomized and sham-operated mice. Numbers within plots indicate percentages of cells in respective areas. (c) Distribution of IgA þ cells in the small and large intestines. Frozen sections were stained with anti-IgA mAb (green) and DAPI (blue). Scale bars, 100 mm. (d) Faeces and sera were collected from appendectomized (n ¼ 12) or sham-operated mice (n ¼ 12) at 4 weeks after conventionalization and GF mice (n ¼ 12), and IgA levels of faeces and sera were determined by ELISA. Each symbol represents an individual mouse, and horizontal bars indicate the mean. Statistical analyses were performed with the Student’s t-test. *Po0.05. NS, not significant.

Article Snippet: IgA levels in the faeces, sera and culture supernatants were determined by a mouse IgA ELISA quantitation kit (Bethyl Laboratories).

Techniques: Isolation, Staining, Enzyme-linked Immunosorbent Assay